Targeted exosomes for the treatment of atherosclerosis, myocardial infarction and stroke

Single-chain antibodies against epitopes associated with vascular dysfunction / injury provide a unique tool for specific targeting of exosomes in vascular repair and regeneration processes e.g. in atherosclerosis, myocardial infarction and stroke. We will construct and test different targeting approaches using single-chain antibodies directed against molecular markers of vascular injury (VCAM) and inflammation (GPIIbIIIa). In vitro proof of successful targeting will be done in flow chamber models, and in vivo models with proof of targeting efficacy in a mouse model of wire-induced endothelial injury. Finally, we will use animal methods of atherosclerosis, plaque rupture, myocardial infarction and stroke to provide proof of concept that targeted exosome therapy can be used to treat these conditions.